This study describes the development and validation for the simultaneous estimation of Etodolac (ETO) And Thiocolchicoside (THIO) by the Simultaneous equation and Absorption ratio method of UV spectroscopy methods. The quantification was achieved at 280 nm (ETO) and 257 nm (THIO) over the concentration range of 1-12 µg/ml for Etodolac (r2=0.9998) and 1-12 µg/ml for Thiocolchicoside (r2=0.9998) by the Simultaneous equation method. Procedure does not require prior separation of components from the sample. LOD values for ETO and THIO were found to be 0.06 μg/ml and 0.04 μg/ml, respectively. LOQ values for ETO and THIO are found to be 0.17 μg/ml and 0.13 μg/ml respectively. The other method involved Q-absorption analysis based on the measurement of absorbance at two wavelengths, i.e λmax of Etodolac (280 nm) and Iso-absorptive point of both drugs (269.2 nm). Beer’s law was obeyed in the concentration range between 1-12μg for both Etodolac and Thiocolchicoside. The results of analysis have been validated statistically and recovery studies carried out in the range 80-120% to confirm the accuracy of the proposed method. The relative standard deviation was found to be <2.0 % in both the methods. The present result shows that the proposed methods can be successfully used for simultaneous determination of the drug content in marketed formulations.
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